Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Direct Human Pan-Granulocyte Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Pan-Granulocyte Isolation Kit isolates functional, highly purified neutrophils, eosinophils, and basophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation, or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.
Cayman Chemical S-Acetyl-L-glutathIon 5g
A derivative of glutathione; more stable in plasma than GSH; converted to GSH by intracellular thioesterases; increases intracellular GSH levels in primary fibroblasts derived from patients with glutathione synthetase deficiency at 50 μM; induces apoptosis in Daudi, Raji, and Jurkat lymphoma cells at 5 mM; inhibits the replication of HSV-1 in human foreskin fibroblasts at 5 and 10 mM; increases survival in a mouse model of HSV-1 infection at 6.25 μg/g per day
STEMCELL Technologies EasySep™ Human Basophil Isolation Kit, For isolating: 1 x 10^9 cells
The EasySep™ Human Basophil Isolation Kit is designed to isolate basophils from a polymorphonuclear cell-rich fraction by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-basophils and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation of fresh peripheral blood.This product replaces the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) for even faster cell isolations.
Biotium Fura-2, AM Ester, 10x100ug
Fura-2, AM ester is an intracellular fluorescent calcium indicator, and the preferred dye for ratiometric imaging microscopy. The membrane-permeant AM ester can be loaded into cells via incubation, but due to limited water solubility, a mild detergent Pluronic F-127 is often used to facilitate cell loading. Fura-2, AM ester itself does not bind Ca2, but intracellular esterases readily hydrolyze it to Fura-2. Upon calcium binding, the excitation maximum of Fura-2 undergoes a blue-shift from 363 nm (no Ca2) to 335 nm (high Ca2) while the emission maximum is relatively unchanged. Measure by flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Properties: Light yellow solid soluble in DMSO; Store at -20°C, protect from light, especially in solution; Kd: 145 nM (22°C, pH 7.2 buffer); Ex/Em = 363/512 nm, e = 27,000 M-1cm-1 (no Ca2); Ex/Em 335/505 nm, e = 35,000 M-1cm-1 (high Ca2); C44H47N3O24; MW: 1001.9; [108964-32-5].
Cell Signaling Technology SimpleChIP Universal qPCR Master Mix 5 ml
SimpleChIP Universal qPCR Master Mix 5 ml
Hampton Research HR2-118/Nucleic Acid Mini Screen - 24 unique reagents, 1.0 ml each plus a 250 ml volume of dehydrant (35% v/v MPD)
HR2-118/Nucleic Acid Mini Screen - 24 unique reagents, 1.0 ml each plus a 250 ml volume of dehydrant (35% v/v MPD). Please note items from this supplier are non-returnable.
Seracare Life Sciences Inc 10% BSA DILUENT/BLOCKING SOL
Convenient antibody diluent/blocking solution concentrate containing 10% bovine serum albumin in phosphate buffer. Compatible with most assay formats.
Cell Biolabs Inc LDL/VLDL and HDL Purification Kit (Ultracentifugation-Free), 10 preps
Cell Biolabs' LDL/VLDL and HDL Purification Kit uses dextran sulfate to selectively and separately purify LDL/VLDL and HDL fractions for various downstream applications. No ultracentrifuge is necessary. Each kit provides sufficient reagents for 10 preps.
Invent Biotechnologies Inc Minute™ Non-Denatured Protein Solubilization Reagent (10 ml)
This item has a minimum qty of 4 per supplier requirements.
This reagent is formulated for dissolving protein pellets using protein kits from Invent Biotechnologies (Cat# Nos. SM-005, SM-005-P, CP-011, NE-013, YM-017, MM-018 and AF-023) and other companies. Solubilized proteins are non-denatured and can be used for ELISA, immunoprecipitation/Co-IP, enzymatic activity determination and other applications.
STEMCELL Technologies EasySep™ Human FITC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human FITC Positive Selection Kit II is designed to isolate cells that are labeled with FITC-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing FITC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human FITC Positive Selection Kit (Catalog #18552).